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Cryopreservative Medium

Japanese

◎ Product outline

Freezing is necessary for cells to prevent transformation after serial passages and to maintain the original properties. Since the technique for cell freezing has been developing, many investigators made by themselves the cryopreservative medium by adding bovine serum and DMSO to the culture media and froze the cells slowly at -1℃/min. Nowadays the cryopreservative medium is widely commercialized and it is easier for investigators to cryopreserve the cells.
 
However, as investigators use more varieties of cells, we have frequently observed reduced viability and impaired proliferation after freeze-thawing. To solve these problems, we optimized the ingredients in the medium and successfully developed new cryopreservative media called LaboBanker series. Consequently LaboBankers make it possible to cryopreserve freezing intolerant cells in the deep freezer.
 
As, in general, the serum-free type of cryopreservative media has less ability to preserve the cells than serum-containing type, applicable cells for serum-free type are limited to freezing tolerant cells. Serum-free LaboBanker 2, however, has almost equivalent performance to serum one, and investigators can select LaboBanker 1 or LaboBanker 2 for the purpose.

◎ Product features

  1. Ready-to-use.
  2. Rapid freezing and long-term storage in a deep freezer (-80℃).
  3. Higher cell viability and rapid proliferation after freezing-thawing.
LaboBanker 1 is containing bovine serum and 10%DMSO.
LaboBanker 1 is applicable to various kinds of cells.
LaboBanker 2 is protein-free and bovine serum-free.
LaboBanker 2 is containing 10%DMSO.
LaboBanker 2 is applicable to various kinds of cells, especially the cells cultured in serum-free media.

◎ Price list

Product name Serum Code Packing Price (yen)
LaboBanker 1 Presence BLB-1 100mL x 1 14,000
BLB-1S 20mL x 4 14,000
LaboBanker 2 Absence BLB-2 100mL x 1 12,000
BLB-2S

20mL x 4

12,000

◎ Experimented cells

A9 HEK293(Serum culture, Serum-free culture) P3・NS-1/1・Ag4.1
C17.2 Hela PC9
C2C12 HepG2 Raji
CEM HL60 Sf9(Serum culture, Serum-free culture)
CHO HOS Sp2/0-Ag14
Colon26 HUT78 T cell hybridoma
COS1 Jurkat THP-1
COS7 LC-2/ad Vero
E.Coli (DH5a, BL21) MG6 WEHI-3
PBMC : Peripheral Blood Mononuclear Cells
  HASMC : Human Aortic Smooth Muscle Cells
  HBEpC : Human Bronchial Epithelial Cells
HNEpC : Human Nasal Epithelial Cells
HMSC-BM : Human Mesenchymal Stem Cells from Bone Marrow   
HPAEpiC : Human Pulmonary Alveolar Epithelial Cells

◎ Protocol

Catalog

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English
LinkIcon
English

◎ Example of Use

Example of LaboBanker 1
Example of LaboBanker 2

◎ Video manual(English)

・LaboBanker Freezing Protocol

 

・LaboBanker Thawing Protocol